TSQ Vantage

Triple Quadrupole Mass Spectrometer

The TSQ Vantage, a triple quadrupole mass spectrometer, includes a Qualitative Enhanced Data scanning function (QED). This proprietary scan function allows the instrument to make data-dependent decisions in response to the sample signals and produce qualitative information in addition to the precise and sensitive quantitative information. The specificity provided by H-SRM followed by QED MS/MS provides uncompromised quantitation performance at low levels followed by a fast, highly-specific full MS/MS scan for confirmation. The patented G2 ion optics (S-lens) provides better transfer (less loss) of more ions to the detector giving better sensitivity, bigger peaks, and better reproducibility in the low end. Additionally, the standard hyperbolic (HyperQuad™ ) quadrupole mass filters reduce chemical noise (H-SRM).

10/8/2009 TSQ Vantage (TQU02360) Installed

Specifications

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    Mass range
  • m/z 10 to 1 500 Da
    Resolution
  • 7500 (FWHM) at m/z 508 of polytyrosine, resolution is continuously adjustable to better than 0.1 Da peak width (FWHM) across the entire mass range
    Scan speed
  • 5,000 amu/sec
    Available activation
  • Collision induced dissociation (CID)
  • 3000 times SRMs (T-SRMs)
  • QED-MS/MS - Simultaneous quantitation and structural confirmation
  • iSRM - Intelligent SRM
  • H-SRM - Highly Selective Reaction Monitoring

Instrument calibrations

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Collision Energy Calculation

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For collision energy calculation check out our MRM/SRM overview page.

Base Collision Energy (CE) Equations (Ref[1]):

  • TSQ Vantage (1.0 mTorr) 2 + peptide: CE = 0.041 x m/z - 3.442
  • TSQ Vantage (1.0 mTorr) 3 + peptide: CE = 0.040 x m/z + 0.773

  • TSQ Vantage (1.5 mTorr) 2 + peptide: CE = 0.030 x m/z + 2.905
  • TSQ Vantage (1.5 mTorr) 3 + peptide: CE = 0.038 x m/z + 2.281

HPLC setup

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the following documents are guidelines to setup the capillary trap/column on our LC-MS systems, this is intended as a supplement to the hands on training required to use the UWPR instruments.

Peptide Standards

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